WebRNA-Seq, polysome profiling and RiboTag libraries were constructed using TruSeq RNA Library Prep Kit v2 (Illumina). Ribo-Seq libraries were constructed as described previously (McGlincy and Ingolia, 2024). Library strategy RNA-Seq Library source transcriptomic Library selection cDNA Instrument model Illumina NextSeq 500 Description tRNA probe WebRibosome protected fragments were isolated as described in McGlincy and Ingolia, Methods, 2024. Ribosome profiling libraries were prepared as described in Ingolia et al., …

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WebHowever, recent genome-wide studies using ribosome profiling (Ingolia et al., 2009, 2012; McGlincy and Ingolia, 2024) indicated that it has functions beyond the unwinding of … WebThisislargelyduetoanormalizationprocedurebias(Chenetal., 2015; McGlincy and Ingolia, 2024) that redistributes translation Molecular Cell 77, 913–925, February 20, 2024 ª … paris rolls royce tour

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WebNicholas J. McGlincy, Nicholas T. Ingolia Department of Molecular and Cell Biology, Center for RNA Systems Biology, California Institute for Quantitative Biosciences, University of … Web2 aug. 2024 · Set up a 3ʹ adapter preadenylation reaction as ( McGlincy and Ingolia, 2024 ). Incubate the reaction at 65°C for 1 h, followed by an incubation at 85°C for 5 min. Add 30 μL RNase-free water and purify the pre-adenylated adapter with the Zymo Oligo Clean & Concentrator kit according to the manufacturer’s protocol. Elute in 6 μL RNase-free water. WebRibosome profiling stands out from these methods. It is a very demanding technique that requires isolation of intact ribosomes, and thus there are multiple additional … paris roubaix cyclo 2022