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Phospho ampk antibody cst

WebThe wells are washed and rabbit anti-APP (T668) antibody is used to detect phosphorylated APP. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of APP (T668) bound. WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody (CST #5005) is ready to ship.

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WebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot. Webo Anti-phospho-AMPK-alpha (Thr172) antibody; Cell Signaling #2531 OR Anti-phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAB; Cell Signalling #2535. o Anti-AMPK-alpha … chkd thrift store norfolk https://thenewbargainboutique.com

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WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). WebApr 11, 2024 · We first found that phospho-AMPK signal widely overlapped with ... Samples were then incubated with 1:200 primary phosphorylated AMPK (pAMPK) antibody in 1.5% goat serum and 1% BSA in PBST at 4°C overnight. ... pERK (1:1000 for Western blotting and 1:2000 for IHC; Cell Signaling Technology, 4370S, lot 12, rabbit), tERK (1:1000; Cell … WebThis antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: … chkd thrift store petersburg va

Phospho-CDK9 (Thr186) Antibody Affinity Biosciences LTD 亲科 …

Category:Phospho-AMPK-alpha (Thr172) Polyclonal Antibody - Thermo …

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Phospho ampk antibody cst

AMPK/pAMPK Western Blot Analysis - Johns Hopkins …

Web® 2015 Cell Signaling Technology, Inc. Orders ff 77-1-CELL (255) orderscellsignal.com Support 77-7-TECH (2) infocellsignal.com Web ff www.cellsignal.com page 2 of 2 9957 For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. WebOct 19, 2024 · Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.

Phospho ampk antibody cst

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Web8 rows · Anti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB. Anti-phospho-AMPK α (Thr172) Antibody MSDS (material safety … WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ).

WebMar 25, 2024 · As an energy sensor, adenosine monophosphate-activated protein kinase (AMPK) plays an important role in activating autophagy by inhibiting mammalian target of rapamycin (mTOR) [ 15 ]. Although AMPK is a key modulator of autophagy, it is less clear whether AMPK is involved in SQSTM1-dependent autophagy to initiate clearance of Keap1 … Web使用 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb(上)或 AMPKα Antibody #2532(下),对未经处理或经寡霉素处理 (0.5 µM) 的 C2C12 细胞提取物进行蛋白质印迹分析。 使用 Phospho-AMPKα (T172) (40H9) Rabbit mAb,对对照(左)或经苯乙双胍处理(右)的石蜡包埋的 NCI-H228 细胞沉淀物进行免疫组织化学分析。 使用 Phospho-AMPKα (Thr172) …

WebImmunogen. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human AMPKα. Antibodies are … WebAMP-Activated Protein Kinases / analysis AMP-Activated Protein Kinases / metabolism* Amino Acid Sequence Animals Caco-2 Cells Cells, Cultured Hepatocytes / metabolism* Humans Male Mass Spectrometry Membrane Proteins / analysis Membrane Proteins / metabolism* Mice Mice, Inbred C57BL Mitochondrial Proteins / analysis

WebAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody Description Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Host species Rabbit Specificity ab23875 recognises the phosphorylated forms of AMPK alpha 1 (T183) and AMPK alpha 2 (T172). Tested applications

WebSnf1 localization was observed in formaldehyde-fixed cells immunostained with anti-phospho-T172-AMPK antibody (1:50, Cell Signalling) and with TRITC-conjugated anti-rabbit antibody (1:100, Life Technologies). For the colocalization experiment, Cdc3-HA was detected with 16B12 monoclonal antibody (1:200, Babco) and with FITC-conjugated anti … grass panicum heavy metalWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … grass parts and functionsWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. chkd thrift store churchland vaWebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Polyclonal Antibody (10HCLC) Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (16) AMPK alpha-1,2 antibodies Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (4) Advanced … chkd thrift store newport news vaWebSep 4, 2014 · Western blot analysis using a phospho-specific antibody revealed a significant increase in phospho-Thr184-AMPK levels in head tissue lysates from ElavGS>UAS-AMPK flies upon RU486 ... Primary antibody, rabbit anti-DILP2 (a generous gift from Dr. Seung Kim), or anti-GFP (Cell Signaling Technology; D5.1, XP) was added 1:250 and incubated … grass pastry tipWebPhospho-AMPKalpha (Thr172) Antibody detects endogenous AMPKα only when phosphorylated at threonine 172. The antibody detects both α1 and … chkd thrift store pick upWebDescription: The AMPK and ACC Antibody Sampler Kit provides an economical means to investigate energy homeostasis and fatty acid synthesis within the cell. The kit includes … chkd thrift store pickup