Phospho nfat flow antibody

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August undefined, 2024

WebAnti-phospho-NFAT1 (pSer54) antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot; Rabbit polyclonal anti-phospho … WebMay 14, 2024 · For both NFAT-mediated GFP ... Cell pellets were resuspended in the residual media by pulsed vortexing. When required, primary antibodies at ~10 µg/ml, diluted in flow wash buffer (100 µl ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at ...

NFAT1 / NFATC2 - LSBio

WebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. howlett hall warkworth

Hypophosphatemia - StatPearls - NCBI Bookshelf

WebThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICFC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341. howlett hierarchy of work motivators

Phospho-NFATC1 (Ser172) Antibody (PA5-64696)

Monitoring of FLT3 phosphorylation status and its response to ... - PubMed

WebProduct Details Antibody Details Format Details Citations & References Brand: BD Transduction Laboratories™ Alternative Name: NF-ATc2; NFATP; KIAA0611 Reactivity: … WebThe costimulatory receptor, CD28, synergizes with the T cell antigen receptor (TCR) to promote IL-2 production, cell survival and proliferation. Despite their profound synergy, the obligatory interdependence of the signaling pathways initiated by howlett hierarchyWebA phosphate test measures the amount of phosphate in your blood. If that sounds too simple, stay tuned for a slight curveball. Phosphate is what you get when you combine the … howlett hill fire department address

"WebThis antibody detects both forms NFAT1 - a ~140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and a ~120 kDa protein in stimulated cells that represents … " - Phospho nfat flow antibody

Phospho nfat flow antibody

phospho-NFAT1 (pSer54) antibody Western N8536

WebAnti-NFAT2 (phospho S237) antibody [EPR2377 (N)] - BSA and Azide free See all NFAT2 primary antibodies Description Rabbit monoclonal [EPR2377 (N)] to NFAT2 (phospho S237) - BSA and Azide free Host species Rabbit … WebHuman Phospho-NFATC1 (S172) Antibody Summary. Detects human NFATC1 in direct ELISAs. Phosphopeptide containing the human S172 …

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WebPhospho-SAPK/JNK (Thr183/Tyr185) Antibody detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. This antibody does not recognize unphosphorylated SAPK/JNK. This antibody may slightly cross-react with phospho-Erk1/2 or -p38 phosphorylated at the homologous residues. WebAnalysis was performed after exposure to drugs (CEP-701 and SU11657), in vitro and in vivo. In response to increasing drug concentrations, there was a linear reduction in P-FLT3. Intracellular flow cytometry analysis correlated with Western blot and XTT assays; flow cytometry data also correlated with FLT3 mutational status.

WebFour NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or …

WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein.

WebJun 27, 2011 · Nuclear factor of activated T cells (NFAT) proteins are Ca 2+ -regulated transcription factors that control gene expression in many cell types. NFAT proteins are …

WebNFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation … howlett hill cemetery onondaga nyWebindicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. SEE ALSO... Additional NFAT Antibodies including NFAT5, NFATc1, NFATc2, NFATc3 and NFATc4 and Cruz Marker™ MW Standards Promotional NFAT Antibodies Phospho-Specific NFAT Antibodies howlett hierarchy of work motivationWebAnti-NFAT2 (phospho S233) antibody [EPR2376 (N)] See all NFAT2 primary antibodies Description Rabbit monoclonal [EPR2376 (N)] to NFAT2 (phospho S233) Host species Rabbit Specificity ab180510 recognizes 7 isoforms Tested applications Suitable for: WB more details Species reactivity Reacts with: Human Immunogen Synthetic peptide. howlett hill fdWebName. Anti-NFAT2 (Phospho-Ser172) Antibody. Description. Rabbit Polycolonal antibody to NFAT2 (Phospho-Ser172) Specificity. NFAT2 (Phospho-Ser172) Antibody detects endogenous levels of NFAT2 only … how let the gods outWebThis antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I). howlett hill fire deptWebProduct Specific Information Phospho-NFATC1 (Ser172) Polyclonal Antibody detects endogenous levels of NFATC1 only when … howlett hill fire department chicken bbqWebAnti-phospho-NFAT1 (pSer54) antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot; Rabbit polyclonal anti-phospho-NFAT1 (pSer54) antibody is used to tag phospho-NFAT1 (pSer54) for detection and quantitation by immunocytochemical and USEN ApplicationsProductsServicesSupport N8536 All Photos(1) howlett home improvement